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Antibody-mediated detection and physical properties of oligonucleotides labelled with multiple internal and terminal
J McClean1, A Davison, M V Rao
1Department of Chemistry, University of Southampton, Highfield, England, U.K.
Biomedical Peptides, Proteins & Nucleic Acids : Structure, Synthesis & Biological Activity
|January 1, 1996
Abstract:
DNP-labelled phosphoramidites have been used to synthesis oligonucleotides with multiple DNP reporter groups. The antibody-mediated detection and the stability of duplexes formed by these labelled oligonucleotides have been studied. A DNP-labelled deoxyuridine triphosphate has also been used to enzymatically incorporate DNP-labels into DNA via the polymerase chain reaction. The use of DNP-labelled primers in the PCR has also been investigated.