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Polymorphous low-grade adenocarcinoma: flow cytometric, p53, and PCNA analysis
R D Kelsch1, T Bhuiya, A Fuchs
1Department of Dental Medicine, Long Island Jewish Medical Center, New Hyde Park, N.Y., USA.
Summary
This study investigated polymorphous low-grade adenocarcinoma of minor salivary glands. While p53 gene mutations and cell proliferation markers were analyzed, no significant correlation was found between these parameters and tumor ploidy.
Area of Science:
- Oncology
- Pathology
- Molecular Biology
Background:
- Polymorphous low-grade adenocarcinoma (PLGA) of minor salivary glands is a recognized entity with reported recurrence and metastasis rates.
- Understanding the molecular and cellular characteristics of PLGA is crucial for predicting its behavior.
Purpose of the Study:
- To evaluate p53 gene product expression and proliferative cell nuclear antigen (PCNA) levels in PLGA.
- To determine the correlation between these markers and flow cytometric analysis of ploidy and proliferative activity.
Main Methods:
- Analysis of 15 formalin-fixed, paraffin-embedded archival cases of PLGA.
- Flow cytometric analysis for ploidy and proliferative activity (S-phase fraction).
- Immunohistochemical staining for p53 protein accumulation and PCNA.
Main Results:
- Three out of 10 analyzed cases exhibited an aneuploid cell line via flow cytometry.
- p53 positive staining was observed in 4 of 15 tumors (5-10% positive cells).
- PCNA staining ranged from 0.5% to 70%, with no consistent correlation to flow cytometry results, except in one case with p53 positivity, high PCNA, and aneuploidy.
Conclusions:
- No significant correlation was found between PCNA-determined proliferation and flow cytometry-derived ploidy in PLGA.
- A single case suggested a potential link between p53 mutation, high proliferation, and aneuploidy, warranting further investigation.