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Related Experiment Videos

High fat diets elevate adipose tissue-derived tumor necrosis factor-alpha activity

C L Morin1, R H Eckel, T Marcel

  • 1University of Colorado Health Sciences Center, Department of Medicine, Denver 80262, USA. Catherine.Morin@uchsc.edu

Endocrinology
|November 5, 1997
PubMed
Summary

High-fat diets increase adipose tissue-derived tumor necrosis factor-alpha (AT-TNF) bioactivity in specific fat pads, but not protein levels. Obesity influences AT-TNF secretion in a fat pad-specific manner.

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Area of Science:

  • Metabolism and Endocrinology
  • Adipose Tissue Biology
  • Inflammation Research

Background:

  • Adipose tissue-derived tumor necrosis factor-alpha (AT-TNF) is linked to insulin resistance and obesity.
  • The bioactivity and environmental influence on secreted AT-TNF protein remain unclear.

Purpose of the Study:

  • To investigate if secreted AT-TNF protein is bioactive.
  • To determine if environmentally induced obesity increases AT-TNF bioactivity and secretion in a fat pad-specific manner.

Main Methods:

  • Male Wistar rats were fed low-fat (LF) or high-fat (HF) diets for 5 weeks.
  • AT-TNF bioactivity was measured in epididymal (EPI), mesenteric (MES), and retroperitoneal (RETRO) fat pads at 1 and 5 weeks.
  • Secreted AT-TNF protein levels were also assessed.

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Main Results:

  • HF diet significantly increased AT-TNF bioactivity in the RETRO pad (P = 0.003) and tended to increase it in the EPI pad (P = 0.07).
  • AT-TNF activity was unaffected in the MES pad.
  • Secreted AT-TNF protein levels decreased in RETRO and EPI pads with diet or time, showing no correlation with bioactivity.

Conclusions:

  • High-fat diets and obesity can modulate AT-TNF bioactivity and secretion.
  • These effects are specific to different fat pads, suggesting localized responses within adipose tissue.