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Multiparameter flow cytometry as a tool to evaluate antipsoriatic therapy

C P Glade1, P E van Erp, J B Boezeman

  • 1Department of Dermatology, University Hospital Nijmegen, The Netherlands.

The British Journal of Dermatology
|February 12, 1998
PubMed
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Multiparameter flow cytometry offers a more accurate assessment of psoriasis severity and treatment efficacy than clinical scores. This method precisely measures epidermal cell changes, distinguishing between antiproliferative and anti-inflammatory effects of therapies.

Area of Science:

  • Dermatology
  • Immunology
  • Biotechnology

Background:

  • Psoriasis severity assessment using clinical scores like the Psoriasis Area and Severity Index (PASI) can be inaccurate.
  • Quantitative analysis of epidermal cell parameters via multiparameter flow cytometry presents a more objective alternative.

Purpose of the Study:

  • To compare clinical assessment with multiparameter flow cytometry for monitoring psoriasis activity.
  • To evaluate the efficacy of antipsoriatic treatments using both methods.

Main Methods:

  • Collected biopsies from 89 psoriasis patients before and after treatment with vitamin D3 analogues and corticosteroids.
  • Analyzed 219 epidermal cell suspensions using triple-labeling for proliferation (DNA dye TO-PRO-3), differentiation (antikeratin 10), and inflammation (antivimentin).

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  • Performed correlation analysis between clinical scores (erythema, induration, scaling) and flow cytometry markers.

    • Main Results:

    • Significant correlations were found between clinical signs and specific cell markers: erythema with vimentin-positive cells, scaling with keratin 10-positive keratinocytes, and induration with basal keratinocytes in S/G2M phase.
    • Flow cytometry clearly differentiated antiproliferative, anti-inflammatory, and keratinization-enhancing effects of treatments, unlike clinical scores.
    • Vitamin D3 analogues showed primarily antiproliferative effects; combination therapy and clobetasol monotherapy significantly improved cell markers.

    Conclusions:

    • Multiparameter flow cytometry is a sensitive tool for evaluating antipsoriatic therapies.
    • It accurately assesses growth inhibition, anti-inflammatory action, and keratinization enhancement.
    • This method provides a more objective and detailed evaluation of treatment response in psoriasis.