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Characterization of changes in gene expression associated with malignant transformation by the NF-kappaB family

O Petrenko1, I Ischenko, P J Enrietto

  • 1Department of Microbiology, State University of New York at Stony Brook, 11794, USA.

Oncogene
|February 12, 1998
PubMed

Insights

Avian bone marrow cells transformed by v-Rel show altered gene expression. This study identifies key genes like MIP-1 chemokines and NF-kB1 involved in v-Rel mediated transformation.

Area of Science:

  • Molecular Biology
  • Oncology
  • Immunology

Background:

  • v-Rel is a viral oncogene that transforms cells.
  • Understanding gene expression changes is crucial for cancer research.

Purpose of the Study:

  • To identify genes whose expression is altered by v-Rel transformation.
  • To investigate the role of these genes in v-Rel mediated oncogenesis.

Main Methods:

  • Conditional v-Rel estrogen receptor chimera (v-RelER) for estrogen-dependent transformation.
  • Subtraction cDNA library construction to identify differentially expressed genes.
  • Gene expression analysis in various cell types (fibroblasts, hematopoietic cells).

Main Results:

  • Identified altered expression of MIP-1 chemokine family genes (mip-1beta, tca3 homologue), sca-2 antigen, and NF-kB1 transcription factor.
  • v-Rel transformation upregulated NF-kB1 and sca-2 in hematopoietic cells.
  • Wild-type v-Rel induced mip-1beta and NF-kB1 in fibroblasts, unlike non-transforming mutants.
  • These genes are also upregulated by c-Rel overexpression, suggesting a shared pathway.

Conclusions:

  • v-Rel transforms cells, in part, by inducing target genes of c-Rel.
  • NF-kB1 and MIP-1beta are implicated in v-Rel mediated transformation.
  • Gene expression profiling provides insights into viral oncogenesis mechanisms.

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