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Genetic diversity and vegetative compatibility among Trichoderma harzianum isolates
I Gómez1, I Chet, A Herrera-Estrella
1Centro de Investigación y Estudios Avanzados, Irapuato, Gto., México.
Molecular & General Genetics : MGG
|February 12, 1998
Summary
Trichoderma harzianum strains show genetic diversity impacting their compatibility. Molecular karyotyping and DNA analysis reveal distinct groups, crucial for understanding biocontrol potential.
Area of Science:
- Mycology
- Molecular Biology
- Plant Pathology
Background:
- Trichoderma harzianum comprises genetically diverse asexual fungal strains.
- Understanding this heterogeneity is key for optimizing biocontrol applications.
Purpose of the Study:
- To investigate the genetic diversity within Trichoderma harzianum isolates.
- To correlate molecular characteristics with isolate compatibility and biocontrol potential.
Main Methods:
- Contour-clamped homogeneous field (CHEF) electrophoresis for chromosomal analysis.
- Randomly amplified polymorphic DNA (RAPD) analysis for genetic fingerprinting.
- Direct confrontation assays to assess isolate interactions.
Main Results:
- Six distinct electrophoretic karyotypes were identified among ten T. harzianum isolates.
- Molecular analyses confirmed distinct groupings and differentiated closely related strains.
- Isolates within the same molecular group exhibited compatible interactions (hyphal fusion), while different groups showed incompatibility.
- Molecular karyotypes strongly correlated with observed isolate compatibility.
Conclusions:
- Molecular karyotyping and RAPD analysis are effective tools for classifying T. harzianum isolates.
- Genetic diversity significantly influences isolate compatibility, a critical factor for biocontrol efficacy.
- This classification system aids in selecting effective T. harzianum strains for plant disease management.