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Sialic acid measurement by a modified Aminoff method: a time-saving reduction in 2-thiobarbituric acid concentration
E L Romero1, M F Pardo, S Porro
1Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Buenos Aires, Argentina.
Journal of Biochemical and Biophysical Methods
|November 14, 1997
Abstract:
Our results show that if the 2-thiobarbituric acid concentration is decreased, its co-precipitation with the chromophore is diminished. Subsequent running of this reaction mixture by high-performance liquid chromatography still allows measurement of Neu-5-Ac in the picomole order, with a substantial time and reactive saving, as compared with the original assay.