Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Matrilin-3 from chicken cartilage

D Belluoccio1, B Trueb

  • 1MEM Institute, Division of Biology, University of Bern, Switzerland.

FEBS Letters
|November 14, 1997
PubMed
Summary

Researchers identified matrilin-3, a novel chicken cartilage protein. Its gene expression is specific to cartilage, suggesting evolutionary links to similar proteins.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A duplication in a patient with 46,XX ovo-testicular disorder of sex development refines the SOX9 testis-specific regulatory region to 24 kb.

Clinical genetics·2017
Same author

The mouse Fgfrl1 gene coding for a novel FGF receptor-like protein.

Biochimica et biophysica acta·2001
Same author

Analysis of the alpha-actinin/zyxin interaction.

The Journal of biological chemistry·2001
Same author

Alternative splicing of the first F3 domain from chicken collagen XIV affects cell adhesion and heparin binding.

The Journal of biological chemistry·2000
Same author

Characterization of a novel protein (FGFRL1) from human cartilage related to FGF receptors.

Genomics·2000
Same author

BSPRY, a novel protein of the Ro-Ret family.

Biochimica et biophysica acta·2000

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Cartilage extracellular matrix composition is complex.
  • Novel protein constituents may play significant roles in cartilage structure and function.

Purpose of the Study:

  • To identify and characterize novel components of chicken cartilage.
  • To understand the molecular basis of cartilage formation and maintenance.

Main Methods:

  • Subtractive cDNA cloning was employed to identify new genes.
  • mRNA expression patterns were analyzed.
  • Protein structure and domains were predicted bioinformatically.

Main Results:

  • A novel chicken cartilage constituent, matrilin-3, was identified.
  • Matrilin-3 is encoded by a 2.2 kbp mRNA restricted to cartilaginous tissues.
  • The predicted 49 kDa protein contains von Willebrand factor A-like and EGF-like domains, with an alpha-helical region suggesting oligomerization.

Conclusions:

  • Matrilin-3 is a novel, cartilage-specific protein in chickens.
  • Its structural similarity to matrilin-1 suggests a common evolutionary origin through gene duplication.

Related Experiment Videos