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Nisin production by Lactococcus lactis using two-phase batch culture

W S Kim1

  • 1Department of Biotechnology, University of New South Wales, Sydney, Australia.

Letters in Applied Microbiology
|November 14, 1997
PubMed
Summary

Two-phase batch culture enhanced nisin production by Lactococcus lactis. Introducing a solvent increased bacterial growth by 21% and nisin yield by 24% compared to traditional methods.

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Area of Science:

  • Microbiology
  • Biotechnology
  • Biochemical Engineering

Background:

  • Nisin is a bacteriocin with significant antimicrobial properties, widely used as a food preservative.
  • Optimizing nisin production is crucial for its cost-effective industrial application.
  • Lactococcus lactis subsp. lactis is a key microorganism for nisin biosynthesis.

Purpose of the Study:

  • To investigate the efficacy of two-phase batch culture for enhanced nisin production.
  • To evaluate the impact of a solvent phase on Lactococcus lactis growth and nisin yield.
  • To determine the partition coefficient of nisin in the chosen solvent system.

Main Methods:

  • Utilized a two-phase batch culture system incorporating phenyl-methyl silicone oil.
  • Cultivated Lactococcus lactis subsp. lactis C2SmPrt-(TnNip) under optimized conditions.
  • Monitored bacterial growth and nisin concentration throughout the fermentation process.
  • Analyzed the partition coefficient of nisin between aqueous and solvent phases.

Main Results:

  • The two-phase batch culture demonstrated a 21% increase in bacterial growth compared to single-phase culture.
  • Nisin production was elevated by 24% in the two-phase system.
  • The partition coefficient of nisin in the solvent varied with the cultivation medium, with a minimum of 1.04.

Conclusions:

  • Two-phase batch culture is a viable strategy for improving nisin production by Lactococcus lactis.
  • The addition of a solvent phase effectively enhances both microbial growth and bacteriocin yield.
  • This approach offers a promising avenue for more efficient industrial-scale nisin manufacturing.

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