Yeast exoglycoproteins produced under NaCl-stress conditions as efficient cryoprotective agents

Six extracellular yeast glycoproteins were prepared from three yeast species in osmotic equilibrium and unequilibrium environments and used as non-penetrating cryoadditives. The glycoprotein secreted by the strain Dipodascus australiensis into the growth medium containing NaCl (8% w/v) was found to be the most effective cryoadditive. It was possible to use this glycoprotein alone (without penetrating agent DMSO) for the cryoprotection of the yeasts studied.