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Characterization of a beta-lactamase from Mycobacterium smegmatis SN2
D Basu1, D V Narayankumar, J Van Beeumen
1Department of Chemistry, Bose Institute, Calcutta, India.
Biochemistry and Molecular Biology International
|November 14, 1997
Abstract:
Beta-lactamases have been reported to be largely responsible for beta-lactam resistance in Mycobacteria. We report the characterization of a cell-associated beta-lactamase from Mycobacterium smegmatis. The enzyme hydrolyzed the "beta-lactamase-stable" oximinocephalosporins. Nitrocefin was the best substrate. 6-Beta-iodopenicillanate, clavulanate and sulbactam were effective inhibitors, whereas the Ki value for aztreonam was high. From its substrate and inhibitor profile, the enzyme appeared to be a cephalosporinase of group 2e.