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Adjuvant-carrageenan-induced inflammation in mice
A M Silván1, M J Abad, P Bermejo
1Department of Pharmacology, Faculty of Pharmacy, University Complutense, Madrid, Spain.
General Pharmacology
|November 14, 1997
Summary
DBA/1 mice effectively model adjuvant-carrageenan-induced inflammation (ACII), showing swelling and histological changes comparable to rats. This strain is suitable for evaluating anti-inflammatory drugs, with histological data aiding assessment.
Area of Science:
- Immunology
- Pharmacology
- Inflammation Research
Background:
- Adjuvant-carrageenan-induced inflammation (ACII) is a model for studying inflammatory conditions.
- Previous studies established ACII induction protocols primarily in rats.
Purpose of the Study:
- To evaluate optimal conditions for inducing ACII in Swiss and DBA/1 mice.
- To compare the ACII response in mice strains with that observed in rats.
- To determine the suitability of DBA/1 mice as an alternative model for anti-inflammatory drug evaluation.
Main Methods:
- Mice were immunized with Freund's complete adjuvant (CFA) via subdermal injection.
- Carrageenan was administered via subplantar inoculation at varying intervals post-immunization.
- Hindpaw and ankle joint swelling were measured, and histopathological analysis of joint tissues was performed.
Main Results:
- DBA/1 mice exhibited significant hindpaw and ankle joint swelling by day 21 post-carrageenan injection.
- Histopathological examination of DBA/1 mice revealed epidermal hyperplasia, leukocyte infiltration, and granuloma formation, mirroring rat responses.
- The timing of carrageenan administration did not significantly alter the observed swelling in DBA/1 mice.
Conclusions:
- DBA/1 mice are a viable model for inducing and studying ACII.
- This mouse strain can be utilized for screening and evaluating the efficacy of anti-inflammatory agents.
- Histological assessment of synovial changes is recommended alongside swelling measurements for comprehensive drug evaluation.