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Gene expression systems in the development of high-throughput screens
1Receptor Biochemistry and Molecular Sciences Department, Glaxo Wellcome Research and Development, Research Triangle Park, NC 27709, USA. jayawickreme-ck@glaxo.com
Current Opinion in Biotechnology
|November 14, 1997
Summary
Automated synthesis and high-throughput screening accelerate the discovery of potential drug molecules. Gene expression systems are crucial for producing proteins needed for assays and developing cell-based screening systems.
Area of Science:
- Drug Discovery and Development
- Biotechnology
- Chemical Synthesis
Background:
- Advancements in automated chemical synthesis and robotic sample handling have expanded compound libraries.
- High-throughput screening (HTS) assays are vital for identifying lead compounds against therapeutic targets.
Purpose of the Study:
- To highlight the importance of HTS assays in drug discovery.
- To emphasize the role of gene expression systems in facilitating HTS.
Main Methods:
- Combinatorial automated chemical synthesis.
- Robotic sample handling and data analysis.
- Development of in vitro biochemical and cell-based high-throughput screening assays.
- Application of gene expression systems for protein production.
Main Results:
- Increased availability of diverse compound libraries for screening.
- Facilitation of rapid identification of selective and potent lead molecules.
- Demonstrated dependence of assay development on effective gene expression systems.
Conclusions:
- Automated synthesis and HTS are key drivers in modern drug discovery.
- Efficient gene expression systems are fundamental for enabling robust biochemical and cell-based assays.
- Integrated approaches in synthesis, screening, and gene expression are essential for therapeutic target identification.