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Related Experiment Videos

Functionally interacting telomerase RNAs in the yeast telomerase complex

J Prescott1, E H Blackburn

  • 1Department of Microbiology, University of California, San Francisco, San Francisco, California 94143-0414 USA.

Genes & Development
|November 14, 1997
PubMed
Summary

Telomerase, a ribonucleoprotein enzyme, adds DNA to chromosome ends. This study reveals that Saccharomyces cerevisiae telomerase contains at least two RNA molecules acting as templates, indicating multiple active sites.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Telomerase is a ribonucleoprotein (RNP) enzyme responsible for maintaining telomere length.
  • Telomerase adds short DNA increments to chromosomal ends in vivo and in vitro.
  • The multimeric state and functional complexity of telomerase have not been fully elucidated.

Purpose of the Study:

  • To investigate the multimeric nature of telomerase from Saccharomyces cerevisiae.
  • To determine if telomerase functions as a multimer and identify the number of active sites.
  • To explore the role of telomerase RNA in DNA polymerization.

Main Methods:

  • Utilizing a previously reported mutant telomerase RNA.
  • Observing the stable binding of the telomerase RNP to its telomeric oligonucleotide reaction product post-polymerization.

Related Experiment Videos

  • Analyzing the templating function of RNA molecules in DNA polymerization.

    • Main Results:

    • Telomerase RNP remains stably bound to the telomeric oligonucleotide product after polymerization.
    • Saccharomyces cerevisiae telomerase complex contains at least two functionally interacting RNA molecules.
    • Both RNA molecules function as templates for DNA polymerization.

    Conclusions:

    • Functional telomerase in Saccharomyces cerevisiae contains at least two active sites.
    • The study demonstrates a novel aspect of telomerase structure and function.
    • Telomerase RNA plays a critical role in DNA polymerization through multiple templating molecules.