Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calmodulin activates phosphatidylinositol 3-kinase

J L Joyal1, D J Burks, S Pons

  • 1Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA.

The Journal of Biological Chemistry
|November 14, 1997
PubMed
Summary

Calcium-dependent protein calmodulin binds to phosphatidylinositol 3-kinase, enhancing its activity. This discovery reveals a new signaling pathway linking calmodulin and phosphatidylinositol 3-kinase in cellular processes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flightless anchors IQGAP1 and R-ras to mediate cell extension formation and matrix remodeling.

Molecular biology of the cell·2020
Same author

IRS2 integrates insulin/IGF1 signalling with metabolism, neurodegeneration and longevity.

Diabetes, obesity & metabolism·2014
Same author

Chin augmentation: Subperiosteal and supraperiosteal implants.

Aesthetic plastic surgery·2013
Same author

Regulation of insulin sensitivity by serine/threonine phosphorylation of insulin receptor substrate proteins IRS1 and IRS2.

Diabetologia·2012
Same author

Olanzapine promotes fat accumulation in male rats by decreasing physical activity, repartitioning energy and increasing adipose tissue lipogenesis while impairing lipolysis.

Molecular psychiatry·2010
Same author

The incidence of enterococci in human feces.

Journal of bacteriology·2010

Area of Science:

  • Cellular Biology
  • Molecular Signaling

Background:

  • Calmodulin is a Ca2+-dependent protein regulating eukaryotic cell processes like cytoskeletal organization and mitogenesis.
  • Phosphatidylinositol 3-kinase (PI3K) is involved in signaling pathways downstream of growth factor receptors.

Purpose of the Study:

  • To investigate the interaction between calmodulin and phosphatidylinositol 3-kinase.
  • To elucidate the functional consequences of this interaction on PI3K activity.

Main Methods:

  • Coimmunoprecipitation assays to detect protein-protein interactions.
  • Affinity chromatography to isolate interacting protein complexes.
  • In vitro and in vivo assays to measure PI3K activity.
  • Use of a calmodulin antagonist (CGS9343B) to assess pathway modulation.

Related Experiment Videos

Main Results:

  • Ca2+/calmodulin directly associates with the Src homology 2 domains of the 85-kDa regulatory subunit of PI3K.
  • This association significantly enhances PI3K activity both in vitro and in intact cells.
  • Calmodulin antagonism with CGS9343B inhibited basal and Ca2+-stimulated phosphatidylinositol phosphorylation.

Conclusions:

  • A novel mechanism for modulating PI3K activity involving calmodulin has been identified.
  • This study establishes a direct functional link between calmodulin and PI3K signaling pathways.
  • The findings provide new insights into fundamental cellular signaling regulation.