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Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

3.1K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
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Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
624
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

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Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
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Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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SDS-PAGE01:27

SDS-PAGE

31.7K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
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Related Experiment Video

Updated: Nov 25, 2025

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
07:58

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

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Capillary electrophoresis

A Sanchez1, A J Smith

  • 1Beckman Center, Stanford University, California 94305-5425, USA.

Methods in Enzymology
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

Capillary electrophoresis (CE) offers high-resolution peptide analysis for purity assessment in peptide synthesis. Combining CE with techniques like HPLC and mass spectrometry enhances synthetic peptide characterization.

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Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
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Last Updated: Nov 25, 2025

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Background:

  • Capillary electrophoresis (CE) is a versatile technique for peptide analysis.
  • CE offers high resolution and charge-based separation, complementing RP-HPLC.
  • Both CE and RP-HPLC are quantitative, suitable for purity assessment.

Purpose of the Study:

  • To highlight the applications of CE in synthetic peptide characterization.
  • To demonstrate CE's utility in monitoring charge-related modifications.
  • To emphasize the synergistic value of CE with other analytical methods.

Main Methods:

  • Capillary electrophoresis (CE) for separation and analysis.
  • Complementary use with Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC).
  • Integration with Mass Spectrometry (MS) and Amino Acid Analysis (AAA).

Main Results:

  • CE effectively monitors charge-related changes like incomplete deprotection or modifications.
  • CE is sensitive to alterations in peptide shape and size.
  • Combined CE analysis provides comprehensive synthetic peptide characterization.

Conclusions:

  • CE is a valuable tool for assessing the purity and integrity of synthetic peptides.
  • CE's complementarity with RP-HPLC enhances its utility in peptide synthesis.
  • The greatest value of CE lies in its combined application with MS, HPLC, and AAA for thorough characterization.