Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Elephantfish proinsulin possesses a monobasic processing site

M A Gieseg1, P A Swarbrick, L Perko

  • 1School of Medical Sciences, University of Otago, Dunedin, New Zealand.

General and Comparative Endocrinology
|November 14, 1997
PubMed
Summary

Researchers discovered a unique single-arginine cleavage site in elephantfish proinsulin, crucial for insulin processing. This finding advances our understanding of hormone evolution and proteolytic processing in vertebrates.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Effect of the Canine ABCB1-1Δ Mutation on Sedation after Intravenous Administration of Acepromazine.

Journal of veterinary internal medicine·2016
Same author

Hypoxia inducible factor (HIF)-2α accelerates disease progression in mouse models of leukemia and lymphoma but is not a poor prognosis factor in human AML.

Leukemia·2015
Same author

A randomized controlled trial of postoperative analgesia following total knee replacement: transdermal Fentanyl patches versus patient controlled analgesia (PCA).

European journal of orthopaedic surgery & traumatology : orthopedie traumatologie·2015
Same author

BSACI guideline for the management of chronic urticaria and angioedema.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2015
Same author

Major change in regiospecificity for the exo-1,3-β-glucanase from Candida albicans following its conversion to a glycosynthase.

Biochemistry·2014
Same author

The pharmacokinetics of methimazole in a novel lipophilic formulation administered transdermally to healthy cats.

New Zealand veterinary journal·2013

Area of Science:

  • * Endocrinology
  • * Molecular Biology
  • * Evolutionary Biology

Background:

  • * Proinsulin undergoes proteolytic cleavage to produce mature insulin.
  • * This processing typically involves specific cleavage sites, often dibasic residues.
  • * The holocephalan Callorhyncus milii (elephantfish) offers a unique model for studying vertebrate evolution.

Purpose of the Study:

  • * To investigate the structure and processing of elephantfish proinsulin.
  • * To identify the cleavage sites involved in converting elephantfish proinsulin to insulin.
  • * To analyze the evolutionary implications of observed cleavage sites.

Main Methods:

  • * Complementary DNA (cDNA) synthesis from pancreatic RNA of Callorhyncus milii.
  • * Polymerase Chain Reaction (PCR) amplification using primers based on elephantfish insulin sequence.

Related Experiment Videos

  • * Amplification of the corresponding genomic DNA fragment for intron analysis.

    • Main Results:

    • * Amplification of an elephantfish proinsulin fragment containing B-chain, C-peptide, and A-chain.
    • * Identification of a novel monobasic cleavage site (single Arg) at the B-chain/C-peptide junction.
    • * The C-peptide/A-chain junction exhibited the expected dibasic cleavage site (-Lys-Arg-).
    • * Flanking sequences supported potential cleavage by PC3 enzymes at the monobasic site.
    • * A 402-bp intron was found at the conserved IVS-2 position in the genomic DNA.

    Conclusions:

    • * The elephantfish proinsulin processing involves a unique monobasic cleavage site, expanding the known repertoire of insulin maturation mechanisms.
    • * This finding provides the first unequivocal evidence of proteolytic processing at a monobasic site in proinsulin.
    • * The conserved intron position suggests ancient origins and functional importance.
    • * This study offers insights into the evolution of insulin processing and its enzymatic regulation across vertebrates.