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Recombineering Homologous Recombination Constructs in Drosophila
Published on: July 13, 2013
Direct cloning of the Brassica S locus by using a P1-derived artificial chromosome (PAC) vector
G Suzuki1, M Watanabe, K Toriyama
1Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Gene
|November 14, 1997
Abstract:
Self-incompatibility of Brassica is regulated by the S locus, which contains several genes including SLG and SRK. We found that both SLG and SRK genes were located at an approx. 80-kb MluI fragment in an S9 haplotype of B. campestris. Therefore, we cloned this MluI fragment into a BssHII site of the P1-derived artificial chromosome (PAC) vector. The utility of the direct cloning method is discussed in this study.
