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Isolation of nuclei for chromatin analysis in fission yeast
1Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Nucleic Acids Research
|February 12, 1998
Abstract:
The methods available for analysis of the chromatin of Schizosaccharomyces pombe are time consuming (>8 h) and/or result in some degradation of the chromatin. Here we report an optimised method for the preparation of spheroplasts and the isolation of nuclei which takes <25 min and is suitable for analysis of chromatin structure by micrococcal nuclease, restriction endonuclease or by immunoprecipitation.
