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Cell activation influences cell staining kinetics

M Sunray1, M Deutsch, M Kaufman

  • 1Schottenstein Cellscan Center for Early Detection of Cancer, Physics Department, Bar-Ilan University, Ramat-Gan, Israel.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|November 14, 1997
PubMed
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Cell stimulation can be detected by monitoring fluorescein fluorescence polarization (FFP). Measuring the rate of fluorescein leakage offers a more sensitive method for observing cell stimulation and cellular responses.

Area of Science:

  • Cellular biology
  • Biophysics

Background:

  • Cellular stimulation is often assessed by changes in intracellular fluorescein fluorescence polarization (IFFP).
  • Existing methods may lack sensitivity in detecting early cellular responses.

Purpose of the Study:

  • To investigate a more sensitive method for detecting cell stimulation.
  • To compare the efficacy of monitoring fluorescein leakage versus polarization changes.

Main Methods:

  • Measurement of intracellular fluorescein fluorescence polarization (IFFP).
  • Quantification of the rate constant for fluorescein leakage from cells.
  • Assessment of fluorescein fluorescence polarization (FFP) in cell suspensions.

Main Results:

  • The rate constant of fluorescein leakage increases significantly with cell stimulation.

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  • This increase in leakage rate is more pronounced than the decrease in polarization.
  • Fluorescein fluorescence polarization (FFP) closely approximates IFFP shortly after cell staining due to minimal leakage.

    • Conclusions:

    • Monitoring fluorescein leakage provides a more sensitive indicator of cell stimulation than traditional polarization measurements.
    • This method offers potential for earlier and more precise detection of cellular responses.
    • The technique is applicable to cell suspensions shortly after the staining process.