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Neutrophil antibodies

D F Stroncek1

  • 1National Institutes of Health, Warren G. Magnuson Clinical Center, Department of Transfusion Medicine, Bethesda, MD 20892-1184, USA.

Current Opinion in Hematology
|November 14, 1997
PubMed
Summary

Neutrophil Fc-gamma-receptor IIIb (Fc gamma RIIIb) gene polymorphisms and alloimmunization in transfusion recipients are better understood. Granulocyte colony-stimulating factor (G-CSF) may treat autoimmune neutropenia by increasing soluble Fc gamma RIIIb.

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Area of Science:

  • Immunology
  • Genetics
  • Hematology

Background:

  • Neutrophil Fc-gamma-receptor IIIb (Fc gamma RIIIb) exhibits genetic polymorphisms.
  • Alloimmunization to neutrophil antigens complicates granulocyte transfusions.
  • Autoimmune neutropenia treatment is an area of active research.

Purpose of the Study:

  • To review recent advances in Fc gamma RIIIb polymorphism.
  • To investigate alloimmunization in granulocyte transfusion recipients.
  • To explore the mechanism of granulocyte colony-stimulating factor (G-CSF) in autoimmune neutropenia.

Main Methods:

  • Analysis of individuals lacking Fc gamma RIIIb.
  • Characterization of Fc gamma RIIIb SH polymorphism.
  • Development of genotyping methods for Fc gamma RIIIB.
  • Production of a monoclonal antibody to NB1 antigen.
  • Evaluation of alloimmunization in granulocyte transfusion recipients.
  • Case report on G-CSF treatment for autoimmune neutropenia.

Main Results:

  • Individuals lacking Fc gamma RIIIb were found to be missing Fc gamma RIIIB and Fc gamma RIIC genes.
  • New methods for Fc gamma RIIIB genotyping (NA1, NA2, SH) were established.
  • Granulocyte transfusion recipients showed alloimmunization to neutrophil-specific and HLA antigens.
  • G-CSF treatment for autoimmune neutropenia may increase soluble Fc gamma RIIIb levels.

Conclusions:

  • Understanding Fc gamma RIIIb polymorphisms is advancing.
  • HLA matching alone is insufficient for granulocyte transfusions in alloimmunized patients.
  • G-CSF may exert therapeutic effects in autoimmune neutropenia partly through soluble Fc gamma RIIIb modulation.

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