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High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
Published on: July 17, 2014
A protein particle vaccine containing multiple malaria epitopes
S C Gilbert1, M Plebanski, S J Harris
1Wellcome Trust Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, UK.
Abstract:
Ty virus-like particles consist of a single protein species that can be produced in yeast. Recombinant Ty-VLPs carrying a string of up to 15 defined cytotoxic T lymphocyte (CTL) epitopes from Plasmodium species prime protective CTL responses in mice following a single administration without adjuvant. Effective processing of epitopes from the string was demonstrated in vitro and in vivo and was not affected by flanking sequences.
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