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Histamine modulates NMDA-dependent swelling in the neostriatum
1Mental Retardation Research Center, University of California-Los Angeles, 90024-1759, USA. ccolwell@npih.mednet.ucla.edu
Brain Research
|August 22, 1997
Summary
Histamine enhances N-methyl-D-aspartate-induced neuron swelling in the brain. This effect is mediated by H2 receptors and potassium channels, suggesting a novel mechanism for modulating N-methyl-D-aspartate receptor function.
Area of Science:
- Neuroscience
- Neuropharmacology
Background:
- N-methyl-D-aspartate (NMDA) receptors play crucial roles in synaptic plasticity and excitotoxicity.
- Histamine is a neuromodulator with diverse functions in the central nervous system.
Purpose of the Study:
- To investigate the effect of histamine on NMDA-induced neuronal swelling in the neostriatum.
- To elucidate the underlying receptor mechanisms and signaling pathways involved.
Main Methods:
- Infrared differential interference contrast videomicroscopy was employed to visualize and quantify neuronal swelling.
- Experiments were conducted on brain slice preparations from developing rats.
Main Results:
- Histamine caused a concentration-dependent potentiation of NMDA-induced neuronal swelling.
- Histamine also enhanced NMDA-dependent swelling induced by electrical stimulation.
- The histamine effect was observed in later developmental stages (postnatal days 7-28) but not early stages (postnatal day 3).
- The enhancement was blocked by H2 receptor antagonist cimetidine and potassium channel blocker tetraethylammonium chloride.
Conclusions:
- Histamine modulates NMDA receptor function in the neostriatum.
- This modulation involves H2 receptor activation and regulation of potassium channels.
- Findings suggest a developmental role for histamine in regulating neuronal excitability via NMDA receptors.