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Related Experiment Videos

Brain neurosteroids during the mouse oestrous cycle

C Corpéchot1, B E Collins, M P Carey

  • 1INSERM U 33, Le Kremlin-Bicêtre, France.

Brain Research
|August 22, 1997
PubMed
Summary

Circadian rhythms significantly impact brain progesterone (PROG) and allopregnanolone (TH PROG) levels in female mice, with internal tissue supply being the main driver, not peripheral sources.

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Area of Science:

  • Neuroendocrinology
  • Steroid Biochemistry
  • Chronobiology

Background:

  • Neuroactive steroids like allopregnanolone (TH PROG) are crucial for brain function.
  • Understanding their regulation throughout the oestrous cycle is key to comprehending female reproductive neurobiology.

Purpose of the Study:

  • To investigate the concentrations of progesterone (PROG) and its metabolites (TH PROG, 5alpha-dihydroprogesterone) in the mouse brain across the oestrous cycle.
  • To determine the influence of circadian rhythms and ovarian cycles on these neurosteroid levels.
  • To explore the relationship between brain and plasma PROG concentrations and their impact on neurosteroid metabolism.

Main Methods:

  • Measurement of brain and plasma PROG, TH PROG, and 5alpha-dihydroprogesterone concentrations in female mice throughout the oestrous cycle.

Related Experiment Videos

  • Analysis of circadian fluctuations in neurosteroid levels.
  • Assessment of 5alpha-reductase activity in brain homogenates.
  • Measurement of pregnenolone sulphate (PREG S) in the brain.

    • Main Results:

    • Significant circadian fluctuations were observed in brain PROG and its metabolites, exceeding cycle-related variations.
    • Brain TH PROG/5alpha-dihydroprogesterone correlated with brain PROG, but not plasma PROG.
    • No correlation was found between endogenous TH PROG/5alpha-dihydroprogesterone and 5alpha-reductase activity.
    • Brain PREG S levels were in phase with plasma PROG, but not brain PROG.

    Conclusions:

    • Circadian and ovarian influences on brain PROG and TH PROG are primarily driven by intra-tissue PROG supply.
    • Peripheral sources play a lesser role in regulating these neurosteroid concentrations in the female mouse brain.
    • The findings highlight the complex interplay between internal and external factors in neurosteroid homeostasis.