Related Experiment Videos
Oxidative stress status in children with nephrotic syndrome
1Department of Laboratory Medicine, Institute of Kidney Diseases & Research Centre, Ahmedabad, India.
Insights
Pediatric nephrotic syndrome is linked to increased oxidative stress, indicated by higher MDA and lower SOD, vitamin C, and vitamin E levels. Supplementation with vitamins C and E may benefit these children.
Area of Science:
- Pediatric Nephrology
- Biochemistry
- Oxidative Stress Research
Background:
- Free radical toxicity is implicated in various clinical conditions.
- Oxidative stress plays a role in pediatric nephrotic syndrome.
- Understanding oxidative stress markers can aid in managing nephrotic syndrome.
Purpose of the Study:
- To investigate oxidative stress status in pediatric nephrotic syndrome patients.
- To compare oxidative stress markers between patients and healthy controls.
- To explore potential clinical implications for managing nephrotic syndrome.
Main Methods:
- Serum levels of malondialdehyde (MDA) and superoxide dismutase (SOD) were measured.
- Serum levels of vitamin C and vitamin E were quantified.
- Patients were classified based on steroid response and relapse frequency.
Main Results:
- Children with nephrotic syndrome exhibited significantly higher MDA levels compared to controls.
- Patients showed significantly lower SOD, vitamin C, and vitamin E levels than controls.
- Steroid-dependent patients displayed the most pronounced oxidative stress fluctuations.
Conclusions:
- Nephrotic syndrome in children is associated with significant oxidative stress.
- Vitamins C and E supplementation is recommended for children with nephrotic syndrome.
- Oxidative stress markers may offer an alternative to renal biopsy in certain clinical scenarios.
Abstract:
In continuation of our work on human stress situation and present day awareness of the role of free radical toxicity in a variety of clinical conditions, oxidative stress status (in terms of serum levels of MDA, scavenging enzyme SOD, vitamins: C and E) has been studied in 45 pediatric patients with nephrotic syndrome (further classified as steroid: responders, frequent/ infrequent relapsers, dependents). The results have been compared with 42 appropriately age healthy children as controls. The salient features of the present study centre around typical observations viz significantly increased levels of MDA (7.92 +/- 2.24 nmol/ml), decreased levels of SOD (1.36 +/- 1.01 U/ml), vitamin C (0.49 +/- 0.17 mg/dl) and vitamin E (0.52 +/- 0.19 mg/dl) in children with nephrotic syndrome as a whole when compared with healthy controls [MDA (4.40 +/- 1.31 nmol/ml), SOD (3.04 +/- 1.83 U/ml), vitamin C (0.60 +/- 0.26 mg/dl) and vitamin E (0.68 +/- 0.25 mg/dl) respectively]. An almost similar trend was encountered in different groups as classified. However, maximum fluctuations were observed in steroid dependents. The present observations appear to be suggestive of alternative guidelines to clinicians in the absence of conventional renal biopsy as the procedure. It is felt that children with nephrotic syndrome should regularly take vitamins C and E from the health point of view.