[Cloning and squencing of human thrombopoietin (hTPO) cDNA and it's expression in COS-7 cells]

Two hTPO cDNA segments (N-terminal and C-terminal) were amplified from human fetal liver mRNA by using separate reverse-transcription PCR reactions, and cloned into pUC19. Their sequences were identical with that previously reported. Then the full length cDNA of hTPO was obtained from the two cDNA fragments, cloned into the shuttle vector pSVK3 and transiently expressed in COS-7 cells. The activity of the expression product was demonstrated with the stimulation of CFU-Meg.