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Published on: June 14, 2016
Microtubule stabilization in pressure overload cardiac hypertrophy
H Sato1, T Nagai, D Kuppuswamy
1Cardiology Section of the Department of Medicine, Gazes Cardiac Research Institute, Medical University of South Carolina, Charleston, South Carolina 29401, USA.
The Journal of Cell Biology
|December 31, 1997
Summary
Microtubule stabilization increases in cardiac hypertrophy, indicated by Glu- and Delta2-tubulin markers. This stabilization, possibly involving microtubule-associated protein 4, contributes to increased microtubule density in pressure overload.
Area of Science:
- Cardiovascular Biology
- Cellular Biology
- Biochemistry
Background:
- Cardiac hypertrophy involves increased microtubule density, potentially due to stabilization.
- Alpha-tubulin undergoes detyrosination and deglutamination, forming Glu- and Delta2-tubulin, markers of stable microtubules.
Purpose of the Study:
- To investigate microtubule stabilization as a mechanism for increased microtubule density in cardiac hypertrophy.
- To characterize the presence of Glu- and Delta2-tubulin in hypertrophied cardiac cells.
Main Methods:
- Generated antibodies for Tyr-, Glu-, and Delta2-tubulin.
- Performed immunofluorescence staining on cardiac cells from control and hypertrophied cats.
- Assessed microtubule stability using drug and cold treatments.
- Quantified microtubule-associated protein 4 (MAP4) mRNA and protein levels.
Main Results:
- Glu- and Delta2-tubulin were significantly increased in right ventricular cardiocytes from pressure-overloaded cats, indicating microtubule stabilization.
- Microtubules in hypertrophied cells showed increased resistance to cold and drugs.
- Microtubule-associated protein 4 (MAP4) expression increased at both mRNA and protein levels in hypertrophied myocardium.
Conclusions:
- Microtubule stabilization is a key feature of pressure overload-induced cardiac hypertrophy.
- Increased microtubule density in hypertrophy may be mediated by microtubule stabilization, potentially involving MAP4.
- Glu- and Delta2-tubulin serve as reliable markers for stable microtubules in cardiac hypertrophy research.
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