Related Experiment Video

Updated: Oct 8, 2026

Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells
07:12

Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells

Published on: September 9, 2021

The activation of the JAK2/STAT5 pathway is commonly involved in signaling through the human IL-5 receptor

N Ogata1, Y Kikuchi, T Kouro

  • 1Department of Immunology, Institute of Medical Science, University of Tokyo, Japan.

International Archives of Allergy and Immunology
|November 18, 1997
PubMed

Insights

Human interleukin-5 (hIL-5) activates the JAK2/STAT5 pathway in eosinophils and TF-1 cells. STAT1 activation was specific to eosinophils, highlighting a common signaling mechanism for hIL-5.

Area of Science:

  • Immunology
  • Cell Signaling
  • Molecular Biology

Background:

  • The Janus kinase (JAK) and signal transducer and activator of transcription (STAT) pathways are crucial for cellular responses to cytokines and growth factors.
  • Interleukin-5 (IL-5) is a key cytokine involved in eosinophil development and activation.

Purpose of the Study:

  • To investigate the activation of the JAK/STAT pathway in response to human interleukin-5 (hIL-5) stimulation.
  • To compare JAK/STAT pathway activation in different hIL-5-responsive cell types, including TF-1 cell lines and primary eosinophils.

Main Methods:

  • Utilized immunoprecipitation and electrophoretic mobility shift assays.
  • Stimulated hIL-5 receptor alpha-subunit (hIL-5R alpha) cDNA-transfected TF-1 cells, butyric-acid-treated YY-1 cells, and peripheral eosinophils with hIL-5.
  • Analyzed tyrosine phosphorylation of JAK2 and activation of STAT5 and STAT1.

Main Results:

  • hIL-5 stimulation induced tyrosine phosphorylation of JAK2 and activation of STAT5 in all tested cell types (TF-h5R alpha, YY-Bu, and eosinophils).
  • STAT1 activation was specifically observed in peripheral eosinophils.
  • JAK2/STAT5 activation represents a common signaling pathway mediating hIL-5 effects across these cells.

Conclusions:

  • JAK2/STAT5 activation is a conserved signaling mechanism downstream of hIL-5 receptor engagement.
  • Differential STAT activation (STAT1 in eosinophils) suggests cell-specific downstream responses to hIL-5.
  • This study elucidates key components of the hIL-5 signaling cascade, relevant for understanding eosinophil biology and related inflammatory conditions.

More Related Videos

Primary Sjogren's Syndrome Associated with Lung Adenocarcinoma: Probing the Potential Common Pathogenic Mechanisms and Experimental Verification10:21

Primary Sjogren's Syndrome Associated with Lung Adenocarcinoma: Probing the Potential Common Pathogenic Mechanisms and Experimental Verification

Published on: September 20, 2024

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

Related Concept Videos

Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a rapamycin-insensitive companion...
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...