Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

EGF receptor phosphorylation is affected by ionizing radiation

T Goldkorn1, N Balaban, M Shannon

  • 1Department of Medicine, University of California, Davis School of Medicine, 95616, USA.

Biochimica Et Biophysica Acta
|November 20, 1997
PubMed
Summary

Ionizing radiation specifically increases tyrosine phosphorylation of the epidermal growth factor (EGF) receptor in A431 cells. This radiation effect on EGF receptor signaling may influence cell growth and apoptosis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Continuous evolving humanoid for advanced cellular models.

SLAS technology·2026
Same author

Time to viral load suppression in antiretroviral-naive and -experienced HIV-infected pregnant women on highly active antiretroviral therapy: implications for pregnant women presenting late in gestation.

BJOG : an international journal of obstetrics and gynaecology·2013
Same author

Transcription factors and th17 cell development in experimental autoimmune encephalomyelitis.

Critical reviews in immunology·2013
Same author

Ceramide mediates nanovesicle shedding and cell death in response to phosphatidylinositol ether lipid analogs and perifosine.

Cell death & disease·2012
Same author

Expression and interdependencies of pluripotency factors LIN28, OCT3/4, NANOG and SOX2 in human testicular germ cells and tumours of the testis.

International journal of andrology·2011
Same author

Developing dual-beam methodologies for the study of heterogeneous polymer-based systems.

Journal of microscopy·2009

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Radiation Biology

Background:

  • Eukaryotic cells exhibit complex responses to ionizing radiation, including cell cycle arrest, DNA repair activation, and cell death.
  • The precise molecular mechanisms underlying these radiation responses remain incompletely understood.
  • The epidermal growth factor (EGF) receptor is a key signaling molecule involved in cell growth and survival.

Purpose of the Study:

  • To investigate the effect of ionizing radiation on EGF receptor phosphorylation and function in A431 cells.
  • To elucidate the specific types of amino acid phosphorylation affected by radiation compared to EGF stimulation.
  • To explore the implications of radiation-induced EGF receptor modulation on cellular processes like growth and apoptosis.

Main Methods:

Related Experiment Videos

  • Exposure of A431 cells and isolated membranes to ionizing radiation and EGF.
  • Phosphoamino acid analysis to determine the specific sites of receptor phosphorylation.
  • Assessment of EGF receptor turnover rates and down-regulation.
  • Inhibition studies using genistein to identify the kinases involved.

Main Results:

  • Ionizing radiation selectively enhances tyrosine phosphorylation of the EGF receptor.
  • EGF stimulation phosphorylates serine, threonine, and tyrosine residues of the EGF receptor.
  • Radiation decreases the turnover rate of the EGF receptor, whereas EGF increases it.
  • Radiation-induced tyrosine phosphorylation is mediated by protein tyrosine kinase activation, as shown by genistein inhibition.

Conclusions:

  • Ionizing radiation modulates EGF receptor signaling through specific tyrosine phosphorylation.
  • The reduced turnover rate of the EGF receptor following radiation may play a role in cellular responses.
  • These findings offer new insights into how radiation impacts EGF receptor function, potentially influencing cell growth and apoptosis.