Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ATP-induced cochlear blood flow changes involve the nitric oxide pathway

T Ren1, A L Nuttall, J M Miller

  • 1Oregon Hearing Research Center, Oregon Health Sciences University, Portland 97201-3098, USA. rent@ohsu.edu

Hearing Research
|November 21, 1997
PubMed
Summary

Adenosine triphosphate (ATP) influences cochlear blood flow (CBF) by causing dose-dependent increases, potentially mediated by nitric oxide pathways and purinoceptors. This study reveals ATP

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Outer hair cell driven reticular lamina mechanical distortion in living cochleae.

Hearing research·2021
Same author

Functional expression of P2X4 receptor in capillary endothelial cells of the cochlear spiral ligament and its role in regulating the capillary diameter.

American journal of physiology. Heart and circulatory physiology·2011
Same author

Effect of capsaicin on potassium conductance and electromotility of the guinea pig outer hair cell.

Hearing research·2010
Same author

Effect of salicylate on KCNQ4 of the guinea pig outer hair cell.

Journal of neurophysiology·2010
Same author

The influence of loud sound stress on expression of osmotic stress protein 94 in the murine inner ear.

Neuroscience·2008
Same author

Spontaneous basilar membrane oscillation and otoacoustic emission at 15 kHz in a guinea pig.

Journal of the Association for Research in Otolaryngology : JARO·2005

Area of Science:

  • Neuroscience
  • Cardiovascular Physiology
  • Otolaryngology

Background:

  • Cochlear blood flow (CBF) regulation is crucial for hearing function.
  • Mechanisms controlling CBF are not fully understood, particularly the role of adenosine triphosphate (ATP).
  • ATP is a known vasoactive agent in other organ systems.

Purpose of the Study:

  • To investigate the effects of ATP on CBF in guinea pigs.
  • To determine if the nitric oxide (NO) pathway is involved in ATP-induced CBF changes.
  • To explore the purinoceptor subtypes mediating these responses.

Main Methods:

  • Anesthetized guinea pigs were used with exposed anterior inferior cerebellar artery (AICA).
  • ATP was perfused into the AICA, and CBF was measured using laser Doppler flowmetry.

Related Experiment Videos

  • The role of the nitric oxide pathway was assessed using N(omega)-nitro-L-arginine methyl ester (L-NAME).

    • Main Results:

    • ATP perfusion induced dose-dependent increases in CBF, reaching up to 220% of baseline.
    • A biphasic response (transient decrease followed by increase) was observed in some animals.
    • L-NAME attenuated ATP-induced CBF increases and enhanced decreases, suggesting NO pathway involvement.
    • Sodium nitroprusside (SNP)-induced CBF changes were unaffected by L-NAME.

    Conclusions:

    • ATP plays a significant role in regulating cochlear blood flow.
    • The biphasic CBF response suggests involvement of both P2x and P2y purinoceptors.
    • ATP-induced increases in CBF are likely mediated by nitric oxide release via endothelial P2y-purinoceptor activation.