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The use of RAPD-PCR as a typing method for Serratia marcescens

A Hejazi1, C T Keane, F R Falkiner

  • 1Department of Clinical Microbiology, Trinity College, Dublin, Ireland.

Journal of Medical Microbiology
|November 22, 1997
PubMed
Summary

Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) is a convenient method for typing Serratia marcescens. This study successfully applied RAPD-PCR to a large collection of S. marcescens isolates, yielding 73 distinct patterns.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Serratia marcescens is a significant nosocomial pathogen.
  • Effective methods for typing S. marcescens are crucial for infection control.
  • Previous typing methods have limitations.

Purpose of the Study:

  • To evaluate random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) as a typing method for S. marcescens.
  • To identify optimal primer combinations for S. marcescens typing.
  • To apply the validated method to a large collection of clinical isolates.

Main Methods:

  • Assessment of various primer combinations for RAPD-PCR.
  • Selection of the most effective primer combination (HLWL-74 and 1254).
  • Application of the chosen RAPD-PCR method to 175 S. marcescens isolates.

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Main Results:

  • The combination of primers HLWL-74 and 1254 provided distinguishable patterns for different serotypes.
  • RAPD-PCR successfully differentiated 175 S. marcescens isolates into 73 distinct patterns.
  • The method demonstrated good reproducibility and effectiveness for a large, diverse collection of isolates.

Conclusions:

  • RAPD-PCR is a convenient and effective method for typing Serratia marcescens.
  • The primer combination HLWL-74 and 1254 is highly satisfactory for S. marcescens differentiation.
  • This study represents the first large-scale application of RAPD-PCR to diverse S. marcescens serotypes.