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Viral promoter expression in CEM-C7 and Jurkat human T-lymphoid cell lines
1Department of Biological Sciences, Keele University, Staffordshire, UK. bia22@keele.ac.uk
Abstract:
The strength of various viral promoters was examined in human T lymphoblastoid cell lines. CMV, RSV and SV40 promoter based-luciferase constructs were transiently transfected into CEM-C7 and Jurkat cells in order to compare promoter strength in each cell type. It was found that the CMV promoter was 10-fold stronger than the RSV promoter in Jurkat cells, but equivalent to the RSV promoter in CEM-C7 cells. Both the CMV and RSV promoters were significantly stronger than the SV40 promoter in Jurkat and CEM-C7 cells. In summary, promoter strengths are cell line specific (Jurkat: CMV > RSV >> SV40; CEM-C7: RSV = CMV >> SV40) and care must be exercised in choosing promoters intended to provide overexpression of chimeric genes.