Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

p53 activity is essential for normal development in Xenopus

J B Wallingford1, D W Seufert, V C Virta

  • 1Department of Zoology, University of Texas, Austin 78712, USA.

Current Biology : CB
|November 22, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Pronephros; a Fresh Perspective.

Integrative and comparative biology·2019
Same author

An under-methylated region in the spacer of ribosomal RNA genes of Lilium henryi.

Plant molecular biology·2013
Same author

Xenopus Dishevelled signaling regulates both neural and mesodermal convergent extension: parallel forces elongating the body axis.

Development (Cambridge, England)·2001
Same author

Online analysis of development.

BioEssays : news and reviews in molecular, cellular and developmental biology·2001
Same author

Calcium signaling during convergent extension in Xenopus.

Current biology : CB·2001
Same author

Regulation of convergent extension in Xenopus by Wnt5a and Frizzled-8 is independent of the canonical Wnt pathway.

The International journal of developmental biology·2001

Blocking the tumor suppressor p53 in Xenopus embryos prevents cell differentiation, leading to developmental tumors. This highlights p53

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cancer Research

Background:

  • The tumor suppressor p53 is crucial for cell cycle regulation and apoptosis in differentiated cells.
  • While p53-deficient mice develop normally, they succumb to neoplasms, suggesting a role in development.
  • Investigating p53's role in vertebrate development requires models beyond mice, like Xenopus laevis embryos.

Purpose of the Study:

  • To investigate the role of p53 in vertebrate embryonic development.
  • To determine the consequences of inhibiting p53 activity during Xenopus development.
  • To explore the potential link between p53 dysfunction and developmental tumors.

Main Methods:

  • Blocking p53 protein activity in Xenopus embryos using dominant-negative human p53 or Xenopus dm-2.

Related Experiment Videos

  • Analyzing the effects on early blastomere differentiation and cell mass formation.
  • Assessing the impact on zygotic gene expression and mid-blastula transition.

    • Main Results:

    • Inhibition of p53 activity blocked differentiation in early Xenopus blastomeres.
    • This resulted in the formation of large cellular masses resembling tumors.
    • Cells with inhibited p53 divided normally but failed to form tissues or differentiate terminally.

    Conclusions:

    • Maternal p53 mRNA and protein are essential for normal Xenopus development.
    • Inhibiting p53 function causes an early block in differentiation, leading to developmental tumors.
    • Xenopus embryos provide a sensitive model for studying p53's role in development and tumors.