Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A novel Cdc42Hs mutant induces cellular transformation

R Lin1, S Bagrodia, R Cerione

  • 1Department of Pharmacology, Cornell University, Ithaca, New York 14853-6401, USA.

Current Biology : CB
|November 22, 1997
PubMed
Summary

The Rho-subfamily GTPase Cdc42Hs regulates cell growth and morphology. A specific mutant, Cdc42Hs(F28L), promotes cell proliferation and transformation, suggesting its role in Dbl-induced oncogenesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Evaluation of quality-control management for pediatric fulminant myocarditis].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2026
Same author

Effects of cotyledon removal on the root growth and physiology properties during early seedling stage of Ricinus communis under salt stress.

Plant biology (Stuttgart, Germany)·2026
Same author

Neuromuscular scoliosis surgery in children.

BJA education·2025
Same author

[Allogeneic hematopoietic stem cell transplantation during the normalization stage of COVID-19 management].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2025
Same author

[Early cellular immune exhaustion in patients with Epstein-Barr virus activation following haploidentical hematopoietic stem cell transplantation].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2025
Same author

Linc-ROR Modulates the Endothelial-Mesenchymal Transition of Endothelial Progenitor Cells through the miR-145/Smad3 Signaling Pathway.

Physiological research·2024

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Oncology

Background:

  • Cdc42Hs, a Rho-subfamily GTPase, regulates critical cellular processes including morphology, polarity, cell cycle, and transcription.
  • The Dbl oncoprotein, a guanine nucleotide exchange factor (GEF), activates Cdc42Hs, and related GTPases like Rac and Rho, when activated, are transforming.
  • Previous attempts to over-express GTPase-defective Cdc42Hs mutants were unsuccessful.

Purpose of the Study:

  • To investigate the role of Cdc42Hs in cell proliferation and transformation.
  • To generate and characterize a novel Cdc42Hs mutant, Cdc42Hs(F28L), that exhibits spontaneous GTP-GDP exchange and retains GTPase activity, mimicking Dbl activation.
  • To determine if Cdc42Hs(F28L) can induce cellular transformation phenotypes.

Main Methods:

Related Experiment Videos

  • Generation of a constitutively active Cdc42Hs mutant (Cdc42Hs(F28L)).
  • Expression of Cdc42Hs(F28L) in cultured fibroblasts.
  • Assays to measure c-Jun kinase (JNK1) activation and filopodia formation.
  • Assessment of transformation hallmarks: reduced contact inhibition, decreased serum dependence, and anchorage-independent growth.

Main Results:

  • Cdc42Hs(F28L) expression activated JNK1 and stimulated filopodia formation in fibroblasts.
  • Stable expression of Cdc42Hs(F28L) resulted in cells with reduced contact inhibition.
  • Cells expressing Cdc42Hs(F28L) showed lower serum dependence for growth and exhibited anchorage-independent growth.

Conclusions:

  • Cdc42Hs plays a significant role in regulating cell proliferation.
  • The Cdc42Hs(F28L) mutant demonstrates functional similarities to Dbl-mediated activation.
  • Cdc42Hs is implicated as a likely physiological mediator of Dbl-induced cellular transformation.