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Potential interfering substances on Falcor-600 and Dax-48 analytical systems
M Fitó1, C Biosca, J M Hernandez
1Department of Clinical Biochemistry, Hospital Germans Trias i Pujol, Ctra. de Canyet s/n, Badalona, Barcelona, Spain.
European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
|November 22, 1997
Summary
Clinical chemistry analyzers can be affected by endogenous interferences like bilirubin, hemolysis, and lipemia. This study assessed these interferences on two analytical systems, providing crucial data for accurate diagnostic testing.
Area of Science:
- Clinical Chemistry
- Analytical Biochemistry
Background:
- Automatic analyzers are increasingly used in clinical chemistry.
- Endogenous interferences can affect the accuracy of diagnostic tests.
Purpose of the Study:
- To evaluate the impact of bilirubin, hemolysis, and lipemia on two automated clinical chemistry analyzers.
- To assess potential interferences in various serum analytes measured by these systems.
Main Methods:
- Two analytical systems, Falcor-600 and Dax-48, were evaluated.
- Interferences were assessed using bovine bilirubin, human red blood cell hemolysate, and Intralipid.
- Measurements included glucose, urea, creatinine, lipids, liver enzymes, and electrolytes.
Main Results:
- Bilirubin, hemolysis, and lipemia were found to interfere with specific analyte measurements.
- The extent of interference varied depending on the analyte and the concentration of the interfering substance.
- Results were obtained following guidelines from the Spanish Society of Clinical Biochemistry and Molecular Pathology.
Conclusions:
- Endogenous substances like bilirubin, hemoglobin, and lipids can cause significant interference in automated clinical chemistry analysis.
- Understanding these interferences is crucial for reliable laboratory diagnostics.
- Method validation should include assessment of common endogenous interferences.