Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Did cyclodextrin glycosyltransferases evolve from alpha-amylases?

G del-Rio1, E Morett, X Soberon

  • 1Instituto de Biotecnologial/UNAM, Cuernavaca, Morelos, Mexico.

FEBS Letters
|November 22, 1997
PubMed
Summary

Cyclodextrin glycosyltransferases (CGTases) likely evolved from alpha-amylases. Combining these enzymes efficiently breaks down starch into glucose, aiding industrial sugar production.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptional regulation of the assT-dsbL-dsbI gene cluster in Salmonella enterica serovar Typhi IMSS-1 depends on LeuO, H-NS, and specific growth conditions.

Journal of bacteriology·2012
Same author

Improving random mutagenesis by purification of the oligonucleotide variants.

Combinatorial chemistry & high throughput screening·2005
Same author

GeConT: gene context analysis.

Bioinformatics (Oxford, England)·2004
Same author

Binding of transcriptional activators to sigma 54 in the presence of the transition state analog ADP-aluminum fluoride: insights into activator mechanochemical action.

Genes & development·2001
Same author

Different strategies to recover the activity of monomeric triosephosphate isomerase by directed evolution.

Protein engineering·2001
Same author

Orthogonal combinatorial mutagenesis: a codon-level combinatorial mutagenesis method useful for low multiplicity and amino acid-scanning protocols.

Nucleic acids research·2001

Area of Science:

  • Biochemistry
  • Enzymology
  • Evolutionary Biology

Background:

  • Alpha-amylases and cyclodextrin glycosyltransferases (CGTases) are crucial enzymes for starch depolymerization.
  • These enzyme families share evolutionary origins, with CGTases potentially evolving from ancestral hydrolases.

Purpose of the Study:

  • To investigate the evolutionary link between alpha-amylases and CGTases.
  • To experimentally determine the adaptive value of CGTase evolution.
  • To explore the synergistic action of alpha-amylases and CGTases in starch saccharification.

Main Methods:

  • Sequence analysis to support the evolutionary hypothesis.
  • Experimental combination of alpha-amylase and CGTase to measure glucose generation from starch.

Related Experiment Videos

Main Results:

  • Sequence analysis provided evidence for the evolution of transferase activity from hydrolase.
  • Combined action of alpha-amylase and CGTase yielded higher glucose production than independent enzyme activity.
  • Demonstrated synergistic saccharification of starch.

Conclusions:

  • CGTases likely evolved from ancestral hydrolases, such as alpha-amylases.
  • CGTases function in concert with alpha-amylases for efficient starch saccharification.
  • This synergistic action has potential applications in industrial processes for producing glucose- and maltose-rich syrups.