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Related Experiment Videos

c-Myb function in fibroblasts

K Bein1, M Husain, J A Ware

  • 1Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.

Journal of Cellular Physiology
|November 25, 1997
PubMed
Summary
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The protooncogene c-myb regulates cell cycle progression and intracellular calcium levels. This study shows myb-dependent transcription impacts cells even without detectable c-myb protein, revealing a broader role in calcium homeostasis.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • The protooncogene c-myb is a nuclear transcription factor.
  • c-myb is implicated in cell cycle regulation through intracellular calcium ([Ca2+]i) concentration.
  • The expression pattern of c-myb is limited, raising questions about its role in other cell types.

Purpose of the Study:

  • To investigate if myb-dependent cell cycle regulation occurs in cells not known to express c-myb protein.
  • To explore the role of c-myb in calcium homeostasis and cell cycle progression in diverse cell types.

Main Methods:

  • Stable transfection of NIH 3T3 fibroblasts with an inducible c-myb dominant-negative construct (pGREMEn).
  • Expression studies in mouse embryonic fibroblasts from c-myb knockout mice.

Related Experiment Videos

  • Measurement of intracellular calcium ([Ca2+]i) levels and cell cycle progression.

    • Main Results:

    • Induced expression of the dominant-negative construct led to G1 cell cycle arrest.
    • This induction also caused a failure to increase late G1 intracellular calcium levels.
    • Fibroblasts from c-myb knockout mice exhibited lower baseline [Ca2+]i levels, which were not further reduced by MEn expression.

    Conclusions:

    • Myb-dependent transcription plays a role in regulating calcium homeostasis and cell cycle progression.
    • This regulation may occur in cells lacking detectable levels of c-myb protein.
    • The findings suggest a broader function for myb in cellular processes beyond its known expression patterns.