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Myoglobin and hemoglobin rotational diffusion in the cell

D Wang1, U Kreutzer, Y Chung

  • 1Biological Chemistry Department, University of California Davis, 95616-8635, USA.

Biophysical Journal
|November 25, 1997
PubMed
Summary

Myoglobin (Mb) mobility in tissues was studied using NMR. Results show Mb facilitates oxygen diffusion within cells, with cellular viscosity impacting its movement differently than hemoglobin (Hb).

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Area of Science:

  • Biophysics
  • Cellular Physiology
  • Biochemistry

Background:

  • Myoglobin (Mb) is crucial for intracellular oxygen transport, but its diffusion properties within cells are not fully understood.
  • Understanding Mb's mobility is key to validating its role in oxygen delivery from the sarcoplasm to mitochondria.
  • Nuclear Magnetic Resonance (NMR) provides a tool to probe molecular dynamics in biological tissues.

Purpose of the Study:

  • To investigate the cellular diffusion properties of myoglobin (Mb) using 1H NMR.
  • To determine the rotational correlation time of Mb within the cellular environment.
  • To compare the cellular microviscosity experienced by Mb and hemoglobin (Hb) and develop methods to differentiate their NMR signals.

Main Methods:

  • Utilized field-dependent transverse relaxation analysis of the deoxy Mb proximal histidyl NdeltaH signal via 1H NMR.

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  • Measured rotational correlation times for Mb in perfused myocardium and compared them to solution values.
  • Analyzed the rotational correlation time of hemoglobin (Hb) in erythrocytes to compare cellular microviscosities.

    • Main Results:

    • The rotational correlation time of Mb in myocardial cells was found to be approximately 1.4 times longer than in solution.
    • This mobility supports the hypothesis that Mb facilitates oxygen diffusion within the cell.
    • Hemoglobin (Hb) exhibited a 2.2-fold increase in rotational correlation time in cells compared to solution, indicating different microviscosities.

    Conclusions:

    • Myoglobin exhibits significant mobility within myocardial cells, consistent with a role in oxygen diffusion.
    • Cellular microviscosity affects Mb and Hb differently, with Mb showing less restricted motion.
    • A relaxation-based NMR strategy can distinguish between overlapping Mb and Hb signals in vivo.