Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optimization of peripheral blood stem cell collection

T Demirer1, W I Bensinger

  • 1Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

Current Opinion in Hematology
|May 1, 1995
PubMed
Summary

Peripheral blood stem cell (PBSC) collections are replacing bone marrow transplants due to faster recovery. However, patient factors affect mobilization, necessitating improved patient identification and mobilization strategies for optimal outcomes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of ABO incompatibility in allogeneic hematopoietic stem cell transplantation.

Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine·2020
Same author

Extracorporeal photochemotherapy in mycosis fungoides.

Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine·2017
Same author

Bendamustine, etoposide and dexamethasone to mobilize peripheral blood hematopoietic stem cells for autologous transplantation in patients with multiple myeloma.

Bone marrow transplantation·2016
Same author

A phase 2 study of bortezomib, cyclophosphamide, pegylated liposomal doxorubicin and dexamethasone for newly diagnosed multiple myeloma.

Blood cancer journal·2016
Same author

A randomized study of melphalan 200 mg/m(2) vs 280 mg/m(2) as a preparative regimen for patients with multiple myeloma undergoing auto-SCT.

Bone marrow transplantation·2015
Same author

Maintenance rituximab after autologous stem cell transplantation in patients with mantle cell lymphoma.

Annals of oncology : official journal of the European Society for Medical Oncology·2015

Area of Science:

  • Hematology
  • Transplant Immunology
  • Cellular Therapy

Background:

  • Peripheral blood stem cells (PBSCs) are increasingly preferred over bone marrow for hematopoietic support due to easier collection and faster recovery kinetics.
  • Growth factors alone can now mobilize adequate PBSCs without prior chemotherapy.
  • Factors like CD34 content, mobilization technique, patient age, marrow disease, and prior treatments influence stem cell yield.

Purpose of the Study:

  • To highlight the challenges in PBSC collection, specifically the high interpatient variability in stem cell yield.
  • To emphasize the need for improved methods to identify patients who may not mobilize sufficient stem cells.
  • To advocate for new strategies to enhance stem cell mobilization in challenging patient subgroups.

Main Methods:

Related Experiment Videos

  • Measurement of CD34 or granulocyte-macrophage colony-forming unit (CFU-GM) content in PBSC collections.
  • Analysis of factors influencing stem cell collection, including mobilization technique, patient age, marrow disease history, prior radiation, and chemotherapy regimens.

Main Results:

  • Mobilization technique, patient age, marrow disease, prior radiation, and chemotherapy regimens are significant factors affecting stem cell collection numbers.
  • High interpatient variation in CD34 collections persists.
  • Certain heavily pretreated patients may fail to mobilize adequate stem cells for rapid engraftment.

Conclusions:

  • Peripheral blood stem cell transplantation is likely to supplant bone marrow transplantation for both allogeneic and autologous transplants within five years.
  • Further research is crucial to refine patient selection and develop novel mobilization strategies.
  • Addressing mobilization challenges is key to maximizing the benefits of PBSC transplantation.