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The effect of cryopreservation on parathyroid cell viability and function
C R McHenry1, D B Stenger, N K Calandro
1Department of Surgery, MetroHealth Medical Center, Case Western Reserve University School of Medicine, Cleveland, Ohio 44109-1998, USA.
American Journal of Surgery
|November 28, 1997
Summary
Cryopreservation significantly reduces live parathyroid cells, impacting calcium and parathyroid hormone regulation. Maximizing tissue quantity may improve cryopreserved autograft success rates.
Area of Science:
- Endocrinology
- Surgical Pathology
- Cell Biology
Background:
- Cryopreserved parathyroid autografts exhibit higher failure rates compared to fresh grafts.
- Understanding cryopreservation's impact on parathyroid tissue is crucial for improving graft viability.
Purpose of the Study:
- To assess cryopreservation effects on live cell yield in parathyroid tissue.
- To evaluate if freezing parathyroid tissue as dispersed cells enhances functional outcomes.
Main Methods:
- Investigated live cell yield and parathyroid hormone (PTH) responses to calcium in bovine parathyroid tissue fragments and dispersed cells before and after cryopreservation.
- Utilized liquid nitrogen freezing for tissue samples.
Main Results:
- Cryopreservation caused a 70%-90% reduction in live cell yield, irrespective of storage duration.
- Dispersed cells showed abolished transient and blunted steady-state calcium (Ca[i]) responses, with elevated PTH secretion post-cryopreservation.
- Parathyroid tissue fragments maintained similar Ca[i] and PTH responses to controls.
Conclusions:
- Cryopreservation diminishes live cell yield independently of storage time.
- Freezing parathyroid cells disrupts normal Ca[i] and PTH regulation.
- Increasing cryopreserved tissue mass may enhance live cell yield and autograft success.