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Expression of syntaxins in rat kidney
B Mandon1, S Nielsen, B K Kishore
1Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-0951, USA.
The American Journal of Physiology
|December 31, 1997
Summary
Syntaxin-3 is found in the basolateral membrane of kidney collecting duct intercalated cells, unlike syntaxin-4. This suggests syntaxin-3 may regulate acid-base transporters and basolateral membrane remodeling.
Area of Science:
- Renal physiology
- Cell biology
- Molecular biology
Background:
- Syntaxin-4 targets aquaporin-2 vesicles to the apical membrane in renal collecting duct principal cells.
- The presence and localization of other syntaxin isoforms in the kidney remain largely uncharacterized.
Purpose of the Study:
- To investigate the intrarenal localization of syntaxin-2 and syntaxin-3.
- To determine the specific cellular and subcellular localization of syntaxin-3 in the renal collecting duct.
Main Methods:
- Reverse transcription-polymerase chain reaction (RT-PCR) on kidney tissues and microdissected nephron segments.
- Immunoblotting using a custom-generated polyclonal antibody against syntaxin-3.
- Immunoperoxidase labeling and immunogold electron microscopy on kidney cryosections.
Main Results:
- Syntaxin-2 and syntaxin-3 mRNA are expressed in the kidney cortex and medulla.
- Syntaxin-3 mRNA is specifically detected in collecting duct cells, thick ascending limb, and vasa recta.
- Syntaxin-3 protein is localized to the basolateral plasma membrane of intercalated cells in the medullary collecting duct.
Conclusions:
- Syntaxin-3 is expressed in the basolateral membrane of renal collecting duct intercalated cells.
- Syntaxin-3 may play a role in targeting acid-base transporters to the basolateral membrane.
- Syntaxin-3 might be involved in basolateral membrane remodeling during acid-base disturbances.