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PDGF-stimulated calcium influx changes during in vitro cell transformation
1Molecular Oncology Program, Cancer Research Center of Hawaii, Honolulu 96813, USA.
Cellular Signalling
|August 1, 1997
Abstract:
Platelet Derived Growth Factor (PDGF)-stimulated intracellular calcium signals were obtained from preneoplastic clones derived from C3H 10T1/2 mouse fibroblasts during a four-week transformation assay. By the end of the assay, when transformed colonies of cells were becoming apparent, the PDGF-stimulated calcium signal had reduced significantly. In addition, whole-cell patch-clamp electrophysiology measurements indicated a marked reduction in expression of T-type calcium channels. These observations demonstrate that PDGF-induced calcium metabolism changes as initiated cells progress to the transformed phenotype.