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Direct enantiomeric separations by high performance liquid chromatography using cyclodextrins

S M Han1

  • 1Department of Pharmaceutical Technologies (UP9200), Smithkline Beecham Pharmaceuticals, Collegeville, PA 19426, USA.

Biomedical Chromatography : BMC
|October 6, 1997
PubMed
Summary

Separating enantiomers, identical molecules, is challenging. Cyclodextrins and modified cyclodextrins are effective chiral selectors in High-Performance Liquid Chromatography (HPLC) for this purpose.

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Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Organic Chemistry

Background:

  • Enantiomer separation is a significant challenge in chemistry due to identical physical and chemical properties.
  • High-Performance Liquid Chromatography (HPLC) has seen increased interest in enantiomeric separation over the past two decades.
  • Chiral stationary phases and chiral additives have advanced significantly, improving separation capabilities.

Purpose of the Study:

  • To review the historical development of cyclodextrins and modified cyclodextrins in HPLC.
  • To explore the diverse applications of cyclodextrins as chiral selectors in enantiomeric separations.

Main Methods:

  • Utilizing cyclodextrins and modified cyclodextrins as chiral selectors.
  • Application as chiral stationary phases in HPLC columns.

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  • Employment as chiral mobile phase additives or chiral counter-ions.

    • Main Results:

    • Cyclodextrins and their derivatives are versatile and widely adopted chiral selectors.
    • These compounds effectively facilitate the separation of enantiomers via HPLC.
    • Demonstrated utility in various analytical and preparative separation contexts.

    Conclusions:

    • Cyclodextrins represent a key advancement in chiral separation technology.
    • Their historical development and varied applications highlight their importance in HPLC.
    • Continued innovation in cyclodextrin-based selectors promises further progress in enantiomeric separation.