Separation of benzodiazepines on a new carbohydrate-based chiral stationary phase for HPLC

Several benzodiazepines were enantioresolved on a new carbohydrate chiral stationary phase based on maltooligosaccharides. The role of organic modifier, ionic strength, pH and temperature are examined and the results are discussed. In general, selectivity and retention were found to decrease with increasing organic modifier concentration. The appearance of two peaks for diazepam suggests that the reported separations are based on separation of conformers and was supported using circular dichroism detection. Counterintuitively, resolution and enantioselectivity were found to improve with increasing temperatures.