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Galactosylation with beta-galactosidase from bovine testes employing modified acceptor substrates
U Gambert1, R Gonzalez Lio, E Farkas
1Institut für Organische Chemie, Universität Hamburg, Germany.
Bioorganic & Medicinal Chemistry
|July 1, 1997
Abstract:
In this study beta1-3 linked analogues of the T-antigen determinant were synthesized in preparative scale by transgalactosylation using beta-galactosidase from bovine testes to give synthetic antigens. Acceptors with modifications of the sugar residue such as alpha-glycosylated spacers, as well as GlcNAc-alphaOR- and 2dGal-alphaOR-substrates opened further possibilities for galactosylation.