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Direct evidence of a connection between autotransplanted microvessel fragments and the host microvascular system
M Nakano1, Y Nakajima, Y Tsuchida
1Department of Plastic and Reconstructive Surgery, Saitama Medical School, Japan.
Abstract:
There are no reports on the autologous transplantation and patency of microvessels in living tissue. We autotransplanted microvessel fragments (Mvf) labeled with DiI-Ac-LDL into the peritoneum and then observed the peritoneum for 7 days postoperatively with a conventional fluorescence or laser scanning confocal microscope. We illustrated a neomicrovascular network of transplanted Mvf labeled with DiI-Ac-LDL in the peritoneum with both a fluorescence and a laser scanning confocal microscope. Furthermore, we demonstrated not only the existence of erythrocytes in the lumina of transplanted DiI-Ac-LDL-labeled Mvf, but also the presence of India ink perfused through the superior mesenteric artery in the lumina of the labeled Mvf. This evidence directly suggests that transplanted Mvf can survive and proliferate to connect adjacent microvascular branches of the superior mesenteric artery in the very early phase of wound healing. Moreover, these findings imply that implantation of Mvf in the microvascular ischemic circulatory tissue might accelerate angiogenesis to reconstitute a new microvascular network connecting to the nearby host microvascular system, which ultimately improves microcirculation.
Insights
Autologous microvessel fragments (Mvf) transplantation into living tissue was successful. These transplanted Mvf survived, proliferated, and formed new vascular networks, improving microcirculation.
Area of Science:
- Regenerative Medicine
- Vascular Biology
- Tissue Engineering
Background:
- Limited data exists on autologous microvessel transplantation and patency in living tissues.
- Understanding microvessel survival and integration is crucial for regenerative therapies.
Purpose of the Study:
- To investigate the survival, proliferation, and vascular integration of autotransplanted microvessel fragments (Mvf) in living tissue.
- To assess the potential of Mvf transplantation to restore microcirculation in ischemic tissues.
Main Methods:
- Autologous microvessel fragments (Mvf) were labeled and transplanted into the peritoneum.
- Post-transplantation observation for 7 days using fluorescence and laser scanning confocal microscopy.
- Assessment of Mvf patency through the presence of erythrocytes and India ink perfusion.
Main Results:
- A neomicrovascular network formed from transplanted Mvf was successfully visualized.
- Evidence of erythrocytes within the lumina of transplanted Mvf confirmed patency.
- India ink perfusion demonstrated functional connection to the host's superior mesenteric artery.
Conclusions:
- Transplanted Mvf can survive, proliferate, and connect with host vasculature in the early stages of wound healing.
- Mvf implantation shows promise for accelerating angiogenesis and improving microcirculation in ischemic tissues.