Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human syntaxin 3 is localized apically in human intestinal cells

M H Delgrossi1, L Breuza, C Mirre

  • 1Laboratoire de Génétique et Physiologie du Développement, Campus de Luminy, Marsaille, France.

Journal of Cell Science
|October 24, 1997
PubMed
Summary

This study identifies human syntaxin 3 as a key protein localized to the apical membrane of intestinal epithelial cells. Its apical targeting signals are conserved across species, including Drosophila melanogaster, highlighting conserved cellular sorting mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Absence of Claudin-10 in the Enamel Organ Alters Its Integrity.

Journal of dental research·2025
Same author

An enhanced workflow for variant interpretation in UniProtKB/Swiss-Prot improves consistency and reuse in ClinVar.

Database : the journal of biological databases and curation·2019
Same author

Cell-to-cell heterogeneity of EWSR1-FLI1 activity determines proliferation/migration choices in Ewing sarcoma cells.

Oncogene·2017
Same author

p63/MT1-MMP axis is required for in situ to invasive transition in basal-like breast cancer.

Oncogene·2015
Same author

Gene Ontology annotations and resources.

Nucleic acids research·2012
Same author

Collective migration of an epithelial monolayer in response to a model wound.

Proceedings of the National Academy of Sciences of the United States of America·2007

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Epithelial Cell Biology

Background:

  • Understanding the sorting of membrane proteins is crucial for epithelial cell function.
  • Syntaxins are key regulators of membrane trafficking and fusion.
  • The specific role of syntaxin 3 in human intestinal epithelial cells remained unclear.

Purpose of the Study:

  • To investigate the molecular mechanisms of apical and basolateral membrane component sorting in human intestinal epithelial cells.
  • To determine the subcellular localization of human syntaxin 3.
  • To assess the conservation of apical targeting machinery for human syntaxin 3 across species.

Main Methods:

  • Cloning of the human homolog of rat syntaxin 3.
  • Subcellular localization studies using endogenous protein and transfected cDNA.

Related Experiment Videos

  • Confocal microscopy in Caco-2 cells.
  • Localization analysis in canine MDCK cells and transgenic Drosophila melanogaster.

    • Main Results:

    • Endogenous human syntaxin 3 localizes to the apical membrane of colon epithelial and Caco-2 cells.
    • Apical localization was confirmed by confocal microscopy in transfected Caco-2 cells.
    • Human syntaxin 3's apical targeting is conserved in canine MDCK cells and Drosophila melanogaster epithelial cells.

    Conclusions:

    • Human syntaxin 3 is localized to the apical membrane of intestinal epithelial cells.
    • The targeting signals and machinery for human syntaxin 3 are conserved across species.
    • This suggests conserved molecular mechanisms for apical protein sorting in epithelial cells.