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Related Experiment Videos

TCR-beta repertoire development in the mouse embryo

H Ema1, A Cumano, P Kourilsky

  • 1Unité 277, INSERM, Département d'Immunologie, Institut Pasteur, Paris, France.

Journal of Immunology (Baltimore, Md. : 1950)
|October 31, 1997
PubMed
Summary

T-cell development begins with biased T-cell receptor (TCR) beta-chain expression, showing preferential V and J segment usage even before selection. This TCR beta-chain repertoire diversifies rapidly within three days during embryonic development.

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Area of Science:

  • Immunology
  • Developmental Biology
  • Molecular Biology

Background:

  • The T-cell receptor (TCR) is crucial for adaptive immunity.
  • Understanding TCR repertoire development is key to immune system function.

Purpose of the Study:

  • To investigate the early stages of TCR beta-chain repertoire formation during embryonic development.
  • To determine the timing of TCR beta and alpha chain rearrangement and diversification.

Main Methods:

  • Analysis of TCR beta-chain V-D-J rearrangements at the messenger RNA level in embryonic thymocytes.
  • Quantification of TCR beta and alpha chain diversity at different gestational ages.

Main Results:

  • Complete TCR beta-chain V-D-J rearrangements were detected by day 14 of gestation.

Related Experiment Videos

  • Preferential usage of specific TCR beta V and J segments was observed early, similar to adults.
  • TCR beta-chain diversity became adult-like by day 16, preceding TCR alpha-chain expression at day 17.

    • Conclusions:

    • TCR beta-chain segment expression is biased from the outset of T-cell repertoire creation.
    • The diversification of the TCR beta-chain repertoire occurs rapidly within three days.
    • Beta-chain diversification precedes alpha-chain rearrangement during thymocyte development.