Two novel lipases from thermophile Bacillus thermocatenulatus: screening, purification, cloning, overexpression, and properties

Related Experiment Video

Updated: Oct 31, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
08:59

Defining Substrate Specificities for Lipase and Phospholipase Candidates

Published on: November 23, 2016

C Schmidt-Dannert1, M L Rúa, R D Schmid

  • 1Institute of Technical Biochemistry, University of Stuttgart, Germany.

Methods in Enzymology
|January 1, 1997
PubMed
Abstract

No abstract available in PubMed .

More Related Videos

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
10:59

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Published on: May 21, 2018

Related Experiment Videos

Last Updated: Oct 31, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
08:59

Defining Substrate Specificities for Lipase and Phospholipase Candidates

Published on: November 23, 2016

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
10:59

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Published on: May 21, 2018

Related Concept Videos

Lipid Catabolism01:25

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
Hyperthermophilic Bacteria01:21

Hyperthermophilic Bacteria

Domain Bacteria includes some unique hyperthermophilic species. They exhibit remarkable adaptations that enable survival in extreme environments.Thermotoga species are rod-shaped, gram-negative, non-sporulating hyperthermophiles that form a sheath-like envelope called a toga. They ferment sugars or starch, producing lactate, acetate, CO₂, and H₂, and can also grow via anaerobic respiration using H₂ and ferric iron. Found in hot springs and hydrothermal vents, over 20% of their genes show strong...

Articles linked to this work by shared authors, journal, and citation graph.

Xylanases targeting unusual glycosidic bonds: Unlocking prebiotic xylooligosaccharides from macroalgae.

Biotechnology advances·2026

Spatial organization of multi-enzyme biocatalytic cascades.

Organic & biomolecular chemistry·2017

Preservation of immobilized bacterial cell-matrix by drying for direct use in microbial sensors.

World journal of microbiology & biotechnology·2014

Use of poly(N-isopropylacrylamide) nanohydrogels for the controlled release of pimaricin in active packaging.

Journal of food science·2012

Recombinant Production of Horseradish Peroxidase Conjugates with Fab Antibodies in Pichia pastoris for Analytical Applications.

Acta naturae·2012

Multiparametric determination of genes and their point mutations for identification of beta-lactamases.

Biochemistry. Biokhimiia·2011

Comparative methods for recombinant production and characterization of 1-deoxy-D-xylulose-5-phosphate synthase from bacterial and apicomplexan sources.

Methods in enzymology·2026

Current methods and applications in the study of 1-Deoxy-D-xylulose 5-phosphate synthase (DXPS).

Methods in enzymology·2026

Thiamine pyrophosphate-dependent enzymes in Toxoplasma gondii and functional significance of an apicoplast-localized pyruvate dehydrogenase.

Methods in enzymology·2026

Biochemical and cryo-EM studies of the pyruvate dehydrogenase complex from native cell extracts.

Methods in enzymology·2026

Synthesis of phosphonate and phosphinate analogs of 2-oxo acids for site-specific inhibition of thiamine-diphosphate-dependent dehydrogenases of 2-oxo acids in vivo.

Methods in enzymology·2026

Use of dihydroxyacetone synthase for the assimilation of methanol in E. coli.

Methods in enzymology·2026

Prussian blue electrocatalyst in bioanalysis: From oxidase biosensors to peroxidase kinetics assays.

iScience·2026

Endothelial KLK10 maintains blood-brain barrier integrity and mitigates ischemic stroke by limiting the cGAS-STING pathway.

Journal of neuroinflammation·2026

Peptide-engineered catalytic and antifouling interface for reliable electrochemical analysis in undiluted biological samples.

Analytica chimica acta·2026

Comparison of two genetic systems for protein production in Cupriavidus necator H16.

Bioresource technology·2026

Advances in Substrate Recognition, Catalytic Mechanisms, and Engineering Applications of Alginate Lyases.

Polymers·2026

Protein Self-Assembly as a Design Principle for Enzyme Immobilisation in Biocatalysis.

International journal of molecular sciences·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us