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Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
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The use of replication-defective retroviruses for cell lineage studies of myogenic cells

D A Fischman1, T Mikawa

  • 1Department of Cell Biology and Anatomy, Cornell University Medical College, New York, New York 10021, USA.

Methods in Cell Biology
|January 1, 1997
PubMed
Abstract

No abstract available in PubMed .

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Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...